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. 2010 Jan 22;6(1):e1000731. doi: 10.1371/journal.ppat.1000731

Figure 7. Phosphatidylserine exposure during SLS induction.

Figure 7

A. Diagram showing the different cell populations detected by staining with AnnexinV and PI. B. Analysis of exposed phosphatidylserine on outer membrane of SLS induced cells. Uninduced cells (-Tet) or cells silenced for 2 and 3 days (+Tet), and cells treated with DTT for 0, 4 12, 20 and 24 hours were reacted with fluorescein isothiocyanate-labeled AnnexinV antibodies (MBL©) and stained with propidium iodide according to the manufacturer's instructions. The cells were analyzed by FACS. The panels represent i. SRα silenced cells; ii. SEC61 silenced cells; iii. SEC63 silenced cells; iv. DTT treated cells. C. AnnexinV - PI staining of SLS induced cells. SRα cells were silenced for 3 days (+Tet). Uninduced and silenced cells were stained with AnnexinV and PI, fixed with 4% formaldehyde and visualized. The exposed phosphatidylserine in propidium iodide negative cells are marked by a white arrow.