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. 2010 Jan 22;6(1):e1000826. doi: 10.1371/journal.pgen.1000826

Figure 2. BRIT1-deficient mice and MEFs showed hypersensitive to γ-irradiation.

Figure 2

(A) BRIT1 −/− mice were hypersensitive to γ-irradiation (IR). BRIT1 +/+, BRIT1 +/− and BRIT1 −/− littermates (n = 30 for each genotype) were exposed to 7 Gy of whole-body IR, and were then monitored for 4 weeks. (B) BRIT1 −/− MEFs were more sensitive to IR. Radiosensitivity of BRIT1 +/+ and BRIT1 −/− MEFs was plotted as the fraction of surviving cells relative to unirradiated cells of the same genotype. (C,D) Enhanced chromosome aberrations in BRIT1 −/− MEFs. BRIT1 +/+ and BRIT1 −/− MEFs were γ-irradiated with 1 Gy, collected at 3 h or 20 h after IR, and subjected to metaphase spread assay. At least 30–35 metaphase spreads were analyzed for chromosome aberrations for each genotype. The main type of chromosome aberrations is chromatid breaks. Representative aberrations in BRIT1 +/+ and BRIT1 −/− MEFs at 3 h post-IR were shown in (D). Arrows represent chromatid breaks.