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ENU-induced mutation frequency (HSV-tk transgene) in ES cells
| ENU concentration |
No. of clones screened |
No. of FIAU- resistant clones |
Loss-of-function mutation frequency |
|---|---|---|---|
| 0.3 mg/ml | 15,200 | 7 | 0.46 × 10−3 |
| 0.5 mg/ml | 9,120 | 9 | 0.99 × 10−3 |
ES cells were selected for FIAU resistance 2 days post ENU treatment. The total number of colonies screened was estimated by plating ES cells on a separate plate not treated with FIAU
