Table 3.
Production of chimeric mice from oxidant-resistant ES cells
| ES clone |
Injection runs |
No. of chimera obtained |
No. of germline- competent chimera |
|---|---|---|---|
| 1E1 | 1 | 2 | 2 |
| 4C11 | 1 | 5 | 2 |
| 5A4 | 3 | 0 | 0 |
Germline transmission was confirmed by genotyping in the F1 progeny. The inheritance of 129X1/SvJ allele of microsatellite markers indicates germline transmission. A total of 20 F1 pups were analyzed from each chimera breeding; failure to transmit 129X1/SvJ genome causes classification of that chimera as germline incompetent