Fig. 2.

Distinct TCR repertoires are induced from vaccination with rV/F-CEA/TRICOM or yeast-CEA. CEA-Tg mice (n = 5) were primed with rV-CEA/TRICOM on day 0 and boosted on days 7 and 14 with rF-CEA/TRICOM. CEA-Tg mice (n = 5) were primed on day 0 and boosted on days 7 and 14 with yeast-CEA. On day 35, mice were killed, spleens were harvested, and splenocytes were pooled. RNA was extracted from splenocytes and underwent RT-PCR using primers specific for Vα (a–c) or Vβ (d–f) genes. Vα profiles for a no treatment, b rV/F-CEA/TRICOM, and c yeast-CEA are shown. Vβ profiles for d no treatment, e rV/F-CEA/TRICOM, and f yeast-CEA are shown. Each gene was quantified by calculating % of total TCR repertoire. Asterisks indicate genes that are uniquely expressed in T cells from mice vaccinated with one vaccine compared to the other