Fig. 3.

Vaccination with rV/F-CEA/TRICOM or yeast-CEA induces distinct cytokine profiles in response to in vitro stimulation with two discrete CEA-specific epitopes. a CEA protein showing the discrete, non-overlapping CEA-526 and CEA-572 epitopes on the A3 loop of domain III. CEA-Tg mice (n = 5) were primed with rV-CEA/TRICOM (solid bars) on day 0 and boosted on days 7 and 14 with rF-CEA/TRICOM; CEA-Tg mice (n = 5) were primed on day 0 and boosted on days 7 and 14 with yeast-CEA (open bars). On day 33, mice were killed and spleens pooled and put into bulk cultures with either b CEA-526 or c CEA-572 peptide for 7 days. IL-2, IL-10, TNF-α, IFN-γ, IL-5, and IL-4 were measured by cytokine bead array (pg/ml/1 × 10⁶ cells) after lymphocytes were restimulated for 24 h with CEA-specific peptide or VSVN peptide control. All data have been normalized to the VSVN peptide control. Please note the differences in the scales of each graph