(A.) DNA copy number was normalized to WT levels. * Indicates significantly different than KO, WT, MnSOD-ala5, and MnSOD-ala9 (p<0.05). ** Indicates significantly different than KO, WT, and MnSOD-ala5 (p<0.05). (B.) DNA was amplified for GAPDH in an intron and exon region. No significant difference was found in GAPDH amplification between the cell lines. (C.) Correlation of mRNA expression to DNA copy number. Expression of MnSOD mRNA correlated to MnSOD DNA copy number (r2=0.29).