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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: J Immunol. 2009 Dec 28;184(3):1251–1260. doi: 10.4049/jimmunol.0901922

Fig. 6. Potential of Sca1+ and Sca1- iB cells.

Fig. 6

(A) Representative example of Sca1, IgM and AA4.1 expression of sorted Sca1+ and Sca1- iB cells from the BM of C57BL/6 mice. >95 % of the cells fell in the sort windows in each experiment. (B) Representative example of the detection of donor cells 4 days after transfer of 5.105 CD45.1+ iB cells into a unconditioned CD45.2+ C57BL/6 host. (C) Expression of CD21 and CD23 on donor-derived cells 4 days after adoptive transfer of and Sca1+ and Sca1- iB cells from congenic CD45.1+ mice into unconditioned CD45.2+ C57BL/6 mice. (D) Fraction of MZ B cells among donor-derived cells 4 days (in one of the 4 experiments, 6 days) after adoptive transfer of Sca1+ and Sca1- iB cells from congenic CD45.1+ into unconditioned CD45.2+ C57BL/6 mice (n = 4, P = 0.006). (E) Average Sca1 expression on FO and MZ B cells generated from adoptively transferred Sca1+ and Sca1- iB cells, calculated as the ratio between geometric mean of Sca1 fluorescence of donor-derived cells and that of host cells of the same phenotype (MZ or FO) in each recipient (n = 4).