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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: J Immunol. 2009 Dec 28;184(3):1251–1260. doi: 10.4049/jimmunol.0901922

Fig. 7. B1 potential of progenitor populations.

Fig. 7

(A) Representative analysis windows for the detection of B1 and B2 cells in the PC of transplanted mice (the example shown is a sublethally irradiated C57BL/6 host transplanted with kit-CLPs). (B) B1/B2 ratio in donor-derived cells in the PC 3 weeks after transfer of 3,000 CLPs, kit-CLPs, MPPs, or LSKF- cells into sublethally irradiated C57BL/6 mice (n = 6, * significantly different from CLPs and MPPs cells). (C) B1/B2 ratio in donor-derived cells in the PC 3 weeks after transfer of 1,500 CD45.1+ CLPs, kit-CLPs or MPPs together with 1,500 CD45.1+CD45.2+ LSKF- cells into sublethally irradiated Rag1-/- mice (n = 4; * significantly different from CLPs and MPPs). (D) Representative example of Sca1 expression on B 1 cells derived from LSKF- cells and from kit-CLPs in a Rag1-/- recipient competitively transplanted with both populations. (E) Nested DNA PCR for the rearrangement of VH11 and VHQ52 in B1 cells isolated by cell sorting from Rag1-/- mice after adoptive transfer of CLPs, kit-CLPs or LSKF- cells.