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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: J Immunol. 2009 Dec 28;184(3):1309–1316. doi: 10.4049/jimmunol.0804162

Figure 4. Ets-1 binds to the majority of ETS motifs predicted in the Th2 ECR.

Figure 4

A) Ets-1 protein/DNA interactions were characterized using a γ–32P-labeled Ets-1 consensus probe. Purified recombinant human Ets-1 (50 ng) was preincubated with an increasing molar excess of the indicated competitors before addition of a high affinity Ets-1 consensus probe. B) EMSA blots were analyzed by densitometry and results were expressed as percent inhibition of the Ets-1 band relative to the uncompeted Ets-1 complex. C) Sequences of the Ets-1 binding site core, the high affinity Ets-1 consensus probe, and the eight conserved ETS motifs predicted within the Th2 ECRs. Bold text indicates nucleotide sites within the Ets-1 core; nucleotides that differ from the defined core sequence are marked by lower case letters.