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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Oncogene. 2009 Nov 9;29(3):368–379. doi: 10.1038/onc.2009.360

Figure 2. The NF1-GRD normalizes DACH1 expression.

Figure 2

(a) Gene expression microarray data of normal human Schwann cells (NHSC), and ST8814 NF1 patient-derived MPNST cells (control) infected with an adenoviral vector expressing GFP (Vector) or the NF1-GRD (GRD). Samples were analyzed in triplicate, and expression is shown relative to the mean of the NHSC samples. Yellow = normal; Red = overexpression; Blue = underexpression. (b) The microarray expression data for the three DACH1 probesets designated by the * in (a) is expressed as fold-change relative to NHSC, showing normalization of DACH1 expression by the NF1-GRD. (c) The NF1-GRD adenovirus blocks Ras/Map-kinase signaling in ST8814 MPNST cells. Ras-GTP was measured in cell lysates after control (Vector) or GRD infection. Ras-GTP, phospo-MEK and phospho-ERK protein levels were each diminished in the presence of the NF1-GRD. (d) Quantification of NF1-GRD effects on DACH1 expression in ST8814 MPNST cells in the gene expression microarray. (e) Confirmation of increased levels of DACH1 expression in response to the NF1-GRD at 18, 24, and 32 hours post-infection using QRT-PCR. Values are fold-change in expression relative to adenoviral vector control.