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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Oncogene. 2009 Nov 9;29(3):368–379. doi: 10.1038/onc.2009.360

Figure 4. Reducing EYA4 expression causes MPNST cell death by necrosis.

Figure 4

shControl (a, b), MPNST-shEYA4.7 (c, d), or MPNST-shEYA4.3 (e,f) cells were fixed and stained with propidium iodide. Flow cytometry analysis of forward scatter (FSC) and side scatter (SSC) in linear amplification was performed along cell cycle analysis are shown in a, c, and e. Identification of the Ao (apoptotic), Go/G, S, and G2/M peaks are shown in b, d, and f. (g) Quantification of cell cycle shows no differences in control or shEYA4.7 or 4.3 cells. (h) A 10% increase in light side scatter mean channel is shown in cells expressing shEYA44.3 or 4.7 as compared to control.