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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Oncogene. 2009 Nov 9;29(3):368–379. doi: 10.1038/onc.2009.360

Figure 5. Reducing EYA4 expression inhibits tumorigenesis.

Figure 5

(a) Gross photographs of nude mice tumors. Black arrows point to flank tumors in mice injected with MPNST cells expressing sh-scramble or shEYA4.3, 4.5, or 4.7. (b) Quantification of tumor volume over time. (c) QRT-PCR showing persistent EYA4 downregulation in small tumors dissected from two mice 59 days after implantation with shEYA44.3, to control for possible loss of shEYA4 expression. (d, f, h, j, l) are photomicrographs of control, and (e, g, i, k, m) are of shEya4.3 tumors (d,e) Low power views of hematoxylin-eosin stain of control (d) tumor and the smaller shEya (e) tumors (40×). The shEya tumor exhibits disproportionate necrosis. ( f, g) Higher power views of each tumor. Larger amounts of necrosis in shEya tumor (f) compared to control (scramble) tumor (g) is apparent (100×). Ki67 stains of control (h) and shEya (i) tumors exhibiting similar degree of proliferation. (400×). Tunel assay of control (j) and shEya (k) tumors exhibiting similar degrees of apoptosis (400×). CD31 stain of control (l) and ShEya (m) tumors exhibiting similar vascularity (400×).