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. 2009 Nov 23;59(2):347–357. doi: 10.2337/db09-0016

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© 2010 by the American Diabetes Association.

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FIG. 3. — KC depletion by GdCl₃ in liver and adipose tissue. Male Wistar rats were depleted of KCs by administration of GdCl₃ as described in research design and methods. Subsequently, liver and adipose tissue were isolated and the presence of macrophages was determined by qRT-PCR assessment of CD68/ED1 and F4/80 mRNA levels (A and B) and counting of CD68/ED1-positive cells after immunohistochemical staining of liver and adipose tissue sections (C–F) as described in research design and methods. Statistical significance is indicated. n = a minimum of six in each group for qRT-PCR and n = 3 for immunohistochemical analysis of CD68/ED1-positive cells. (A high-quality digital representation of this figure is available in the online issue.)