FIG. 8.

The effects of TNFα depletion on hepatocyte lipid metabolism and insulin-stimulated PI 3-kinase activity. Hepatocytes and KCs were isolated from rat livers, plated, and cultured as described in research design and methods. Subsequently, the effects of the indicated exposures on fatty acid oxidation and triglycerides in the absence or presence of a TNFα-neutralizing antibody were assessed as described in research design and methods (A). In separate experiments, hepatocytes were incubated with 100 nmol/l insulin for 10 min after similar exposures to those in (A), proteins extracts were then prepared, and IRS-1–associated PI 3-kinase activity was assessed as described in research design and methods (B). Data are presented as means ± SE. Statistical significance is indicated. n = a minimum of six for each experimental condition, except for triglycerides (n = 3).