FIG. 4.

Different response of bTKO β-cells to mitochondria (staurosporine) or ER stress (thapsigargin)-mediated apoptosis. Islets isolated from lox/lox and bTKO mice were incubated for 24 h in the presence or absence of staurosporine (0.5 μmol/l) (A and B) or thapsigargin (25 μmol/l) (C and D), and β-cell apoptosis was assessed by TUNEL. More than 12 islets and more than 700 β-cell nuclei were analyzed per group. Representative images are shown. Insulin, red; nuclei, blue; TUNEL-positive nuclei, green (white arrows). Bars represent means ± SEM. (A high-quality digital representation of this figure is available in the online issue.)