FIG. 4.

PGE₂ production and the effects of PGE₂ and pertussis toxin on insulin secretion in mfat-1–expressing INS-1 cells and islets. A: Measurement PGE₂ concentrations in the medium incubated with INS-1 cells (preinfected with Ad-mfat-1 or Ad-lacZ) in the presence or absence of AA (3 or 9 μmol/l) for 30 min. B: The concentrations of PGE₂ in the medium incubated for 30-min with isolated islets from mfat-1 transgenic or wild-type mice. C: Islets isolated from the transgenic or wild-type mice were preincubated with 10 μmol/l PGE₂ in the presence or absence of 50 ng/ml PTX for 12 h before the study of glucose-stimulated insulin secretion. The results are means + SEM from three independent repeats with each condition carried out in eight replicates and six islets in each replicate. ^#P < 0.01, *P < 0.05 when mfat-1 group compared with corresponding control group.