TABLE 1.

Analysis of n-6 and n-3 PUFA composition

	Total n-6	Total n-3	n-6/n-3 ratio
Mouse tissues (%)
Tails
Wild type	23.71 ± 0.32	5.21 ± 0.21	4.76 ± 0.23
mfat-1	8.90 ± 1.79*	13.72 ± 0.87*	0.66 ± 0.17*
Islets
Wild type	32.39 ± 0.05	6.36 ± 0.02	5.10 ± 0.01
mfat-1	8.37 ± 0.05*	21.16 ± 0.01*	0.39 ± 0.01*
Virally infected (%)
INS-1
Adenovirus carrying lac-Z cDNA	13.13 ± 0.83	2.29 ± 0.49	5.54 ± 1.78
Adenovirus carrying mfat-1 cDNA	4.12 ± 0.65*	8.26 ± 0.27*	0.50 ± 0.09*
Wild-type
Adenovirus carrying lac-Z cDNA	28.42 ± 0.30	7.73 ± 0.11	3.68 ± 0.01
Adenovirus carrying mfat-1 cDNA	18.85 ± 0.12*	15.48 ± 0.07*	1.22 ± 0.01*

Data are means ± SD. The tissues (50 mg of tail samples or ∼200 islets) were collected from the mfat-1 transgenic or wild-type control mice. Fatty acid analysis was also performed on the INS-1 or the wild-type islets infected with adenoviral vectors carrying either mfat-1 or lacZ cDNA. The compositions of n-6 or n-3 PUFAs were expressed using relative percentages, i.e., the distribution areas of n-3 or n-6 PUFA peaks divided by the total peak areas of all detectable saturated and unsaturated free fatty acids (from the same sample) resolved from the gas chromatography column. In the transgenic tissues, the modest drop in relative percentages of n-3 plus n-6 PUFAs corresponded to a minor percentage increase in some other detectable fatty acid species. However, the increase was primarily reflected in the most dominant peak of oleic acids (tails, 26.9% [wt] vs. 33.0% [mfat-1]; islets, 17.6% [wt] vs. 22.8% [mfat-1]). The specificity of mfat-1 enzymatic activity primarily caused the changes of n-6 and n-3 PUFAs. n = 4.

*P < 0.001 when mfat-1 group compared with corresponding control (wt or lacZ) group.