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. 2009 Oct 29;59(2):407–415. doi: 10.2337/db09-0694

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© 2010 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 2. — nTregs of type 1 diabetic subjects display impaired IL-2R signaling. A: Level of expression of CD25, CD122, and CD132 in the CD4⁺CD25⁺ population of control (n = 17) and type 1 diabetic (n = 11) subjects from Fig. 1C was determined using Quantum R-PE MESF beads. B: Thawed PBMCs from a representative control and type 1 diabetic subject were stimulated with 100 IU/ml IL-2 for 20 min prior to fixation and staining with CD4, CD25, and pSTAT5(Y694). Dashed lines are treatment with media alone and solid lines are treatment with IL-2. C: Multiple control (n = 15) and type 1 diabetic (n = 17) subjects were stimulated as in B. Bars show means and symbols represent individual subjects. Analysis of pSTAT5 was performed by gating on live CD4⁺CD25⁺ T-cells. Statistical significance was determined using an independent Student t test.