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. 2009 Oct 29;59(2):407–415. doi: 10.2337/db09-0694

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© 2010 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 4. — CD4⁺ T-cells of type 1 diabetic subjects display diminished responsiveness to IL-2. Thawed PBMCs from control subjects were stimulated with 100 IU/ml IL-2 for 10 min prior to fixation and staining for CD4, CD25, and pSTAT5(Y694). Analysis was performed by gating on total live CD4⁺ T-cells and comparing response to media alone versus cytokine stimulation. A: Staining for one representative sample is shown. B: The frequency of CD4⁺ T-cells that were pSTAT5(Y694)⁺ in response to IL-2 was determined for control (n = 59) and type 1 diabetic (n = 33) subjects. Bars represent means and symbols represent individual subjects. C: Control (n = 12) and type 1 diabetic (n = 13) subjects were assayed for pSTAT5(Y694) in response to stimulation for 10 min with 200 pg/ml IL-15 or 40 pg/ml IL-7. Bars represent means ± SEM. D: MFI fold increase of pSTAT5(Y694) in STAT5⁺ CD4⁺ T-cells of thawed PBMCs from control (n = 12) and type 1 diabetic (n = 14) subjects was determined by comparing pSTAT5(Y694) MFI after stimulation with IL-2, IL-7, and IL-15, or media alone. Bars show means and symbols represent individual subjects. All P values were determined using an independent Student t test.