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. 2009 Oct 29;59(2):407–415. doi: 10.2337/db09-0694

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© 2010 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 5. — Diminished IL-2 responsiveness is a stable phenotype and intrinsic property of CD4⁺ T-cells of type 1 diabetes. A: Thawed PBMCs from control (n = 18) and type 1 diabetic (n = 20) subjects were stimulated with 100 IU/ml IL-2 or 200 pg/ml IL-15 for 10 min prior to fixation and staining for CD4, CD25, and pSTAT5(Y694). Linear regression was performed to determine the relationship between pSTAT5 responses in the same cells after stimulation with either IL-2 or IL-15. Trend lines represent linear regression of control (R² = 0.3713, P = 0.0073) and type 1 diabetes (R² = 0.2003, P = 0.0478) data. B: Cells isolated from the same individual but at different dates were assayed as described (in A) for response to IL-2. Sample collection dates varied from 5 months to 6 years. The number of months between sample collection did not correlate with SD or coefficient of variation for the control subjects, type 1 diabetic subjects, or all samples combined as analyzed by linear regression.