FIG. 1.

SAA3 and MCP-1 expression is stimulated by specific SFAs and inhibited by specific PUFAs. 3T3–L1 adipocytes were differentiated in 5 or 25 mmol/l glucose and cultured for 7 days in the same medium with various FFAs (250 μmol/l). Total RNA was isolated and analyzed by multiplex real-time RT-PCR using SAA3-specific (A) or MCP-1–specific (B) primers and probes and normalized to GAPDH. C: Conditioned media were analyzed by immunoblot using anti-SAA3 antibody. *P < 0.001 vs. 5 mmol/l glucose control. **P < 0.001 vs. 25 mmol/l glucose control.