FIG. 2.

HA content and HAS2 gene expression are increased by high glucose conditions but not SFAs and attenuated by arachidonate, EPA, and DHA. 3T3–L1 adipocytes differentiated in 5 or 25 mmol/l glucose were cultured for 7 days in the same media with or without various FFAs (250 μmol/l). A: Cell lysates that included cell-associated extracellular matrix were harvested and analyzed for HA content by ELISA. B: Total RNA was isolated and analyzed by multiplex real-time RT-PCR using HAS2 specific primers and probes and normalized to GAPDH. *P < 0.001 vs. 5 mmol/l glucose control. **P < 0.001 vs. 25 mmol/l glucose control.