Table 1.

Proportion of tumors with at least 2-fold increase in protein expression

Day-5	Day-10	Day-15	Day-20	Day-25	Day-30
ALDH	0/6	0/6	6/6	3/6	0/6	0/6
Shh	0/6	0/6	6/6	0/6	0/6	0/6
Notch	0/6	0/6	4/6	0/6	0/6	0/6
p63	0/4	nd	4/4	nd	nd	0/4
BCRP	0/4	nd	4/4	nd	nd	0/4
bcl-2	0/4	nd	2/4	nd	nd	0/4
Grp78	0/4	nd	0/4	nd	nd	4/4

After normalization to β-actin, a band density at least double the mean of the non-castrated control samples in each reaction was considered to be an overexpression. nd: not done.