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. 1998 Dec 22;95(26):15659–15664. doi: 10.1073/pnas.95.26.15659

Figure 1.

Figure 1

Disruption of Y1-R gene in mice. (A) Partial restriction map of the allele of wild-type Y1-R and Y5-R genes (Top), the targeting construct (Middle), and the predicted homologous recombinant allele (Bottom). The closed and open boxes indicate coding and noncoding regions in the exons, respectively. Pertinent restriction enzyme sites are noted (B, BamHI; H, HindIII; X, XhoI; K, KpnI). P1, P2, and P3 indicate the positions of the PCR primers used in genotyping. (B) Southern blot analysis of DNA from the tails of Y1-R+/+, Y1-R+/−, and Y1-R−/− mice using the 3′ probe indicated in A. Wild-type (7.5 kbp) and recombinant (5.5 kbp) KpnI-digested fragments were identified. (C) Northern blot analysis of Y1-R and Y5-R mRNA expressions in Y1-R+/+ and Y1-R−/− mouse brain. Y1-R and Y5-R mRNAs were identified by using Y1-R and Y5-R cDNAs as probes, respectively (14, 15).