Figure 7.

(A) Northern blot analysis of UCP1, UCP2, and UCP3 mRNAs in BAT, WAT, and skeletal muscle. Total RNA was isolated from the BAT, WAT, and skeletal muscle of Y1-R^+/+ and Y1-R^−/− female mice (18–45 weeks old) kept at 23°C by using the guanidinium thiocyanate method. Five micrograms of total RNA per lane was used for these experiments. (B) Bar graph showing the comparison of mRNA levels of UCP1, UCP2, and UCP3 in WAT, BAT, and skeletal muscle of Y1-R^+/+ and Y1-R^−/− female mice. Data are presented as the mean ± SE of estimates for six female mice for WAT and BAT and three mice for skeletal muscle. A two-tailed unpaired Student’s t test was used for statistical analysis.