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Human iPS cells can differentiate into cardiomyocytes in co-culture with END-2 cells. Immunofluorescent staining of contracting areas with antibodies against cardiac structural proteins β-myosin heavy chain (A, red) or α;-actinin (B, C, red) and cardiac transcription factor NKX 2.5 (A, B, green). At higher magnifications, cells stained for α;-actinin show the characteristic striated pattern of cardiomyocytes (C). All cells were co-stained with a nuclear dye (Dapi, blue).
