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. 2010 Jan 19;120(2):498–507. doi: 10.1172/JCI39447

Figure 2. NLP gene amplification in human tumor.

Figure 2

(A) Total RNA (0.5 μg) isolated from 30 paired lung tumor tissues and normal adjacent tissues were used for semi-quantitative RT-PCR assays. (B) DNA was extracted from the lung cancer samples and their normal adjacent tissues (See Figure 1C). DNA (20 μg per specimen) was digested with EcoRI and hybridized with NLP probe. The estimated size of the hybridized NLP band was approximately 7.7 kb. In addition, the digested DNA was also hybridized with GAPDH probe, which was included as an internal control. (C) Human lung carcinomas and their normal adjacent tissues from A were used for FISH analysis. In addition, multiple human cancer cell lines (lung cancer, cervical cancer, and esophageal cancer cells) were included for analysis of NLP gene copy numbers. NLP-hybridizing loci were visualized as red spots, and the hybridizing signals for chromosome 20–specific centromeric probe were green. Original magnification, ×400.