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. 2010 Jan 19;120(2):498–507. doi: 10.1172/JCI39447

Figure 5. Characterization of Nlp transgenic mice.

Figure 5

(A). NLP construct for generation of transgenic mice. (B) PCR analysis of tail DNA of NLP transgenic founders (Tg) and wild type (C) mice. M, DNA marker; —, negative control. (C) Immunofluorescent examination of newborn tail tips. The tail tips were obtained from 2-day-old mice and were observed by fluorescent microscopy. Original magnification, ×10. (D) Western blot analysis of different organs of NLP transgenic mice. Nlp expression was detected in skin, thymus, heart, testicle, breast, lung, and kidney of transgenic mice.