Figure 2. cDCs confer protection in I/R.

CD11c-DTR mice were administered PBS or DT via i.p. injection. Twelve hours later, mice received an i.v. injection of 1 × 10⁷ WT cDCs or PBS and then underwent sham laparotomy or I/R. (A) Serum ALT levels and (B) representative liver H&E staining (original magnification, ×100) are shown at 12 hours after sham procedure or liver I/R. Nonviable patches of ischemic lobes are demarcated by dashed lines. CD11c-DTR mice were pretreated with DT or PBS 12 hours earlier and then subjected to sham laparotomy or 12 hours of I/R. (C) Serum cytokines levels were measured or (D) ischemic liver CD45⁺ NPCs were harvested and cultured overnight in media prior to measurement of supernatant cytokines. Data represent mean ± SEM (A, C, and D). Data in all panels are representative of at least 3 independent experiments; n = 4–6 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001.