Figure 3. cDC-mediated protection in liver I/R is TLR9 and IL-10 dependent.

CD11c-DTR mice were administered DT via i.p. injection. Twelve hours later, mice received an i.v. injection of either PBS or 1 × 10⁷ WT, Tlr9^–/–, or Il10^–/– cDCs just prior to I/R. (A) Serum ALT levels and (B) cytokines levels were measured 12 hours later. (C) WT or Tlr9^–/– (both CD45.2) cDCs were injected into WT (CD45.1) recipients just prior to sham laparotomy or I/R. Twelve hours later, ischemic liver CD45⁺ NPCs were isolated and cultured in media in the presence of Brefeldin A. Intracellular IL-10 production by CD45.2⁺CD11c^hi hepatic cDCs was assayed 6 hours later and is expressed as the percentage within each gated region based on IL-10 isotype control antibody staining. The bar graph represents pooled data from 3 independent experiments. Data represent mean ± SEM and are representative of at least 2 independent experiments; n = 4–6 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001.