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. 2010 Jan 11;120(2):508–520. doi: 10.1172/JCI40045

Figure 6. β-catenin signaling inhibits Kras-induced reprogramming of acini into PanINs.

Figure 6

(A, E, and I) H&E staining of tamoxifen-stimulated, caerulein- or PBS-treated ElaCreERT;β-cateninexon3/+;LSL-KrasG12D or ElaCreERT;LSL-KrasG12D mice. (B, F, and J) CK19/Alcian blue staining. Note that normal ducts in PBS-treated ElaCreERT;β-cateninexon3/+;LSL-KrasG12D mice are strongly CK19 positive but Alcian blue negative (B). (C, G, and K) β-catenin (green), DAPI (blue) immunofluorescent labeling. β-catenin accumulation is rare in PBS-treated ElaCreERT;β-cateninexon3/+;LSL-KrasG12D mice (asterisk, C). Nuclear localization is noted by cyan overlap of green/blue channels (K). (D, H, and L) phospho-p42/p44 staining. Original magnification, ×400 (A, B, D–F, H–J, and L). Scale bars: 50 μm (C, G, and K).