Fig. 5. Treg development in IL2Rβ^−/− and IL2Rβ^−/− x IL7Rα^−/− mice.

A. Thymus and spleen were harvested from 4–5 week old LMC, IL7Rα^−/−, and IL2Rβ^−/− x IL7Rα^−/− mice. Cells were stained with antibodies to CD4, CD8 and Foxp3 to identify Tregs. Shown are flow cytometry plots of thymus (top panel) or spleen cells (bottom panel) gated on CD4⁺ T cells. A representative example of 6 independent experiments is depicted. B. Shown are bar graphs representing total numbers of CD4⁺Foxp3⁺ Tregs in the thymus (left panel) and spleen (right panel). Error bars represent standard error of the mean; n= 6 IL7Rα^−/− and 6 IL2Rβ^−/− x IL7Rα^−/− mice; p-values were calculated using two-tailed students t-test.