Fig. 6. Expression of IL2Rβ, IL7Rα and IL15Rα on CD4⁺Foxp3⁺ Tregs from IL2^−/− and IL2Rβ^−/− mice compared to wild type littermate controls.

Splenocytes were isolated from 4–5 week old LMC, IL2^−/− and IL2Rβ^−/− mice and stained with antibodies for CD4, CD8, and Foxp3 to identify splenic Tregs. Shown are CD4⁺Foxp3⁺ gated cells stained for IL2Rβ (left panels), IL7Rα (middle panels) and IL15Rα (right panels). Gray histograms represent staining of CD4⁺Foxp3⁺ cells with isotype control antibody. Solid lines represent histograms of CD4⁺Foxp3⁺ T cells from IL2^−/− (top panel) or IL2Rβ^−/− (bottom panel) mice; broken lines represent staining of CD4⁺Foxp3⁺ Tregs from WT LMC mice. A representative example of 3 independent experiments is depicted (n= 6 IL2^−/− and 3 IL2Rβ^−/− mice).