Table 1. PIK3CA Mutation Frequency.
Results from exon 9 and exon 20 mutational screens from the P024, RAD2222 POL and Z1031 trials. For the P024 and RAD2222 trials, total sample numbers are from the cases in which mutation status of both PIK3CA exons were determined. PIK3CA KD and HD mutation status was determined in all POL and Z1031 cases.
| PIK3CA Mutations in P024 | Cases | Domain | Exon |
|---|---|---|---|
| G1624A:E542K | 8 | Helical | 9 |
| G1633A:E545K | 8 | Helical | 9 |
| T1568C:L523S | 1 | Helical | 9 |
| G1573A:E525K | 1 | Helical | 9 |
| T1592C:L531P | 1 | Helical | 9 |
| Total in HD (%) | 18/152 (11.8%) | ||
| A3140G:H1047R | 29 | Kinase | 20 |
| Total in KD (%) | 29/163 (17.8%) | ||
| Total | 45/153 (29.4%) | ||
| PIK3CA Mutations in RAD2222 | Cases | Domain | Exon |
|---|---|---|---|
| G1633A:E545K | 9 | Helical | 9 |
| G1624A:E542K | 2 | Helical | 9 |
| C1636A:Q546K | 2 | Helical | 9 |
| A1634G:E545G | 1 | Helical | 9 |
| Total in HD (%) | 14/102 (13.7 %) | ||
| A3140G:H1047R | 19 | Kinase | 20 |
| A3140T:H1047R | 2 | Kinase | 20 |
| A3127G:M1043V | 1 | Kinase | 20 |
| C3139T:H1047Y | 1 | Kinase | 20 |
| C3145G:G1049R | 1 | Kinase | 20 |
| C3145T:G1049A | 1 | Kinase | 20 |
| 3204_3205ins A:NSTOP>KLKRSTOP | 1 | Kinase | 20 |
| Total in KD (%) | 26/102 (25.5%) | ||
| Total | 38/97 (39.2%) | ||
| PIK3CA Mutations in POL | Cases | Domain | Exon |
|---|---|---|---|
| G1624A:E542K | 2 | Helical | 9 |
| G1633A:E545K | 4 | Helical | 9 |
| C1370G:N457K | 1 | C2 | 7 |
| Total in HD (%) | 7/79 (10%) | ||
| A3140G:H1047R | 13 | Kinase | 20 |
| A3140T:H1047L | 4 | Kinase | 20 |
| G3068T:R1023L | 1 | Kinase | 20 |
| Total in KD (%) | 18/79 (22.8%) | ||
| Total | 25/79 (31.7%) | ||
| PIK3CA Mutations in Z1031 | Cases | Domain | Exon |
|---|---|---|---|
| G1624A:E542K | 3 | Helical | 9 |
| G1633A:E545K | 4 | Helical | 9 |
| Total in HD (%) | 7/69 (10.1%) | ||
| A3140G:H1047R | 12 | Kinase | 20 |
| Total in KD (%) | 12/69 (17.4%) | ||
| Total | 19/69 (27.5%) | ||