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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: Nat Genet. 2009 Mar 1;41(4):482–487. doi: 10.1038/ng.335

Figure 4. BMP-6 interacts with HJV.Fc.

Figure 4

BMP-6 alone, HJV.Fc alone, or BMP-6 in combination with HJV.Fc were incubated in solution. (Upper panel) BMP-6 bound to HJV.Fc was precipitated with protein A beads, and the eluted protein complex was analyzed by SDS-PAGE, followed by Western blot with BMP-6 antibody under reducing conditions. The band migrating at ~18 kDa present only in the lane containing BMP-6 in combination with HJV.Fc corresponds to the predicted size of BMP-6 monomer (arrow). (Lower panels) As a control to demonstrate input proteins, solution aliquots prior to Protein-A pull-down were also analyzed by Western blot with anti-Fc antibody and anti-BMP-6 antibody under reducing conditions. Control Westerns revealed the predicted bands migrating at 18 kDa for BMP-6 monomer and 75 and 60 kDa for HJV.Fc4. A background band at ~19 kDa was present in the lanes containing HJV.Fc probed with BMP-6 antibody suggesting nonspecific crossreactivity of this antibody with a component of the HJV.Fc solution.