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. Author manuscript; available in PMC: 2011 Jan 18.
Published in final edited form as: Chem Res Toxicol. 2010 Jan;23(1):99–107. doi: 10.1021/tx900301r

Figure 5.

Figure 5

A) A native PAGE gel analysis showing double stranded cleavage of a 5'-end-labeled 47-bp oligomer duplex (Pu47) by treatment with leinamycin. B) Protective effect of methoxyamine on leinamycin-induced double-stranded DNA breaks. A 5'-end-labeled Pu47 was treated with leinamycin in the presence of increasing concentrations of methoxyamine. To determine true DNA strand breakage sites, the DNA samples were dissolved in 20 µL of a neutral formamide dye and were electrophoresed in a 16% denaturing PAGE gel without heat denaturation.