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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: J Immunol. 2008 May 1;180(9):5927–5934. doi: 10.4049/jimmunol.180.9.5927

FIGURE 7.

FIGURE 7

Increased expression of Ifi202 in B6.Nba2 mice is associated with reduced expression levels of E2F1 and inhibition of E2F1-mediated transcription of target genes.

(A) Sub-confluent cultures of B6 or B6.Nba2 MEFs were transfected with E2F-luc-reporter plasmid (2.5 μg) and pRL-TK plasmid (0.5 μg) using a calcium phosphate transfection kit as described in methods. Cells were harvested after 44 h to assays for the firefly and Renilla luciferase activities as described in methods. Normalized relative luciferase activity is shown.

(B) Total RNA was isolated from splenocytes from age-matched (10 weeks) B6 (lane 1) or B6.Nba2 (lane 2) female mice and steady state levels of mRNAs were analyzed by semi-quantitative RT-PCR using a pair of primers specific for the indicated individual genes.

(C) Total cell extracts were prepared from splenocytes isolated from age-matched (10 weeks) B6 (lane 1) or B6.Nba2 (lane 2) female mice and steady state levels of proteins were analyzed by immunoblotting using antibodies specific for the indicated proteins.

(D) Total cell extracts were prepared from splenocytes isolated from age-matched (10 weeks) B6 (lanes 1 and 2) or B6.Nba2 (lanes 3 and 4) female mice and steady state levels p73 and actin proteins were analyzed by immunoblotting using specific antibodies.