Figure 7.

Structural dependence of conformational dynamics in HP67. Exchange broadened ¹³C^α and ¹³C methyl groups are mapped onto the structure of HP67. (a) Atoms are colored to correlate with the classifications depicted in Figs. 5 and 6; thus, green, blue and brown spheres represent residues that are fully disordered, retain native-like interactions and have non-native-like conformations in the intermediate, respectively. As can be seen, two regions of residual interactions are maintained the intermediate: (i) at the interface of the N- and C-terminal subdomains and (ii) in the vicinity of His41. (b) ¹³C^α and ¹³C methyl groups affected by the fast process with k_ex = (4.2 ± 0.5) × 10⁴ s⁻¹ are mapped to the structure and color coded to reflect the magnitude of exchange broadening. Atoms for which R_ex > 2.5 s⁻¹are colored red, atoms for which 1 s⁻¹ < R_ex < 2.5 s⁻¹ are orange, and atoms for which R_ex < 1 s⁻¹are colored yellow.