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. 2008 Aug 30;15(2):273–283. doi: 10.1089/ten.tea.2008.0055

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Copyright 2009, Mary Ann Liebert, Inc.

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FIG. 2. — Extracellular matrix (ECM) and osteogenic gene expression levels in mesenchymal stem cells (MSCs) cultured on poly(lactide-co-glycolide) (PLGA). (A) MSCs were cultured on spin-coated PLGA substrates for 1, 7, 14 and 28 days in normal growth medium or osteoinductive medium (OS medium). Type I collagen, fibronectin, runt-related transcription factor 2 (Runx2), bone sialoprotein, and osteocalcin gene expression levels were assessed by RT-PCR at each timepoint. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNA expression was also examined as an internal control. Representative images of ethidium bromide–stained agarose gels are shown. (B–E) After scanning densitometry, type I collagen (B), fibronectin (C), Runx2 (D), and bone sialoprotein (E) gene expression levels were normalized to corresponding GAPDH expression levels, and the ratios were compared to OS medium day 28 condition. Statistical comparisons were made at each time point between the two different media conditions (*p < 0.05, ***p < 0.001).

FIG. 2. — Extracellular matrix (ECM) and osteogenic gene expression levels in mesenchymal stem cells (MSCs) cultured on poly(lactide-co-glycolide) (PLGA). (A) MSCs were cultured on spin-coated PLGA substrates for 1, 7, 14 and 28 days in normal growth medium or osteoinductive medium (OS medium). Type I collagen, fibronectin, runt-related transcription factor 2 (Runx2), bone sialoprotein, and osteocalcin gene expression levels were assessed by RT-PCR at each timepoint. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNA expression was also examined as an internal control. Representative images of ethidium bromide–stained agarose gels are shown. (B–E) After scanning densitometry, type I collagen (B), fibronectin (C), Runx2 (D), and bone sialoprotein (E) gene expression levels were normalized to corresponding GAPDH expression levels, and the ratios were compared to OS medium day 28 condition. Statistical comparisons were made at each time point between the two different media conditions (*p < 0.05, ***p < 0.001).