FIG. 3.

Coating poly(lactide-co-glycolide) (PLGA) substrates with purified extracellular matrix (ECM) proteins alters alkaline phosphatase (ALP) activity. Non-tissue culture dishes spin-coated with a 1% solution of 85:15 PLGA were incubated with a purified ECM protein solution [type I collagen (100 μg/mL), fibronectin (5 μg/mL), or vitronectin (2 μg/mL)] or kept uncoated overnight at 4°C. Mesenchymal stem cells were cultured on these substrates, and ALP activity was assessed after 14 and 21 days of culture. The ALP values for all of the ECM-coated PLGA substrates were significantly higher than the uncoated control surface (***p < 0.001).