Table 1.

Primers and Conditions for Reverse Transcriptase Polymerase Chain Reaction

Gene	Primers (F = forward; R = reverse)	Accession Number	Amplicon Size (bp)	Annealing Temperature (°C)
Type I collagen	F: 5′-CTGGTGATGCTGGTCCTGTT-3′	NM_000088	620	65
	R: 5′-CATGTAGGCCACGCTGTTCT-3′
Fibronectin	F: 5′-AGGAGACCACATGAGACT-3′	X02761	355	52
	R: 5′-TGCCTCTCACACTTCCACT-3′
Runt-related transcription factor 2/core binding protein A-1	F: 5′-GGAATGCCTCTGCTGTTATG-3′	NM_001024630	581	65
	R: 5′-TGCCTGGCTCTTCTTACTGA-3′
Bone sialoprotein	F: 5′-CTCAATCTGTGCCACTCACT-3′	NM_004967	355	62
	R: 5′-TCCTCCTCCTCTTCTGAACT-3′
Osteocalcin	F: 5′-CACCGAGACACCATGAGAGC-3′	NM_199173	270	65
	R: 5′-GTGGTCAGCCAACTCGTCAC-3′
Glyceraldehyde-3-phosphate dehydrogenase	F: 5′-AAGGTCGGAGTCAACGGATT-3′	NM_002046	443	65
	R: 5′-CAGGAGGCATTGCTGATGAT-3′