Figure 5.
Inhibition of Notch1 signaling reduces cell proliferation after MCAO in adult rat brain SVZ. BrdU-positive cell counts in SVZ of ischemic brain were reduced by inhibitors of Notch1 (Jagged-Fc) (A)or γ-secretase (DAPT) (B). Data are means±s.d. (n = 4 to 6 per group). (C) Immunostaining shows the decrease in BrdU-positive cells in the SVZ 72 h after MCAO. (D) Double immunostaining shows BrdU labeling of nuclei in DCX-positive cells. (E) Tuj1-positive cell counts in SVZ of ischemic brain were not significantly altered by inhibitors of Notch1 (Jagged-Fc) (left panel) or γ-secretase (DAPT) (right panel).