Table 3.
Primers used for genes amplification and sequencing.
| Locus | Putative gene Product | Locus position* | Gene size (bp) | Sequence length (bp) | Primers** | Primer sequence 5'-3' |
|---|---|---|---|---|---|---|
| aroC | Chorismate synthase | 568275 | 1094 | 433 | 43f | TGGGGCGAAAGCCACGGTCTG |
| 740r | CCTTCACGGCGTTGATCGACA | |||||
| dnaK | Heat shock protein 70 kDa | 818851 | 1910 | 534 | 591f | CACGCTCGCCTGGAAGACGC |
| 1865r | GGGAACGACCAACTCCTGCGT | |||||
| 1777r | TCGCTTACGGTCTGGACAAG | |||||
| gap | Glyceraldehyde-3- phosphate dehydrogenase | 1259699 | 1007 | 578 | 138f | TCTGCGTTATGACAGCGTTC |
| 940r | AAGCCCATTCATTGTCGTA | |||||
| 866r | GAAGACCGAGGAATGGGAGT | |||||
| omp25 | 25 kDa outer membrane protein | 2714010 | 641 | 390 | 188f | ACGCGGAACTTGCTTTCGTCG |
| 649r | GCGCACTCTTAAGTCTCTCG | |||||
| recA | Recombinase A | 2079528 | 1085 | 490 | 1f | ATGTCTCAGAATTCATTGCGA |
| 988r | CTGACGAAGCGTGGTTTCGAT | |||||
| 697r | ATACGGCGAATATCGAGACG | |||||
| rpoB | Beta sub-unit RNA Polymerase | 2046339 | 4133 | 501 | 457f | ATCGTTTCGCAGATGCACCG |
| 1449r | GACATACGTTCCTTGATCGCG | |||||
| 1119r | TGACGCGATAGATGTCGAAC | |||||
| trpE | Anthranilate synthase | 1671911 | 2195 | 564 | 15f | TGCGGATAGCGAGATATTCCA |
| 1486r | GCCGATGCCTTCAATTCGGT | |||||
| 659r | GTTGCCGTGCGAGACCAT | |||||
(f) forward primer; (r) reverse primer. Primers in bold were used for gene sequencing in both directions. (*) gene start codon position on the chromosome I sequence of O. anthropi ATCC 49188T (accession number: CP000758). (**) primer denomination corresponded to its hybridization region in the gene according to the complete genome sequence of O. anthropi ATCC 49188T