Figure 2.

RT-PCR for ERβ mRNA in +/+ and −/− tissues. (A) Gel electrophoresis of the products. The arrowheads indicate the bands corresponding to full-length ERβ mRNA and the β-actin control mRNA. The marker is labeled m. A lane loaded with PCR product in the absence of reverse transcriptase is labeled “−RT”. (B) Diagrammatic representation of the RT-PCR products (see text) from wild-type and mutant mRNA (ERβ-KO₁, ERβ-KO₂, and ERβ-KO₃) derived by using the primers indicated by the black arrows. The top line (Protein) shows the domains of the protein corresponding to the exons 1–9. A/B, N-terminal domain; C, DNA-binding domain; D, hinge domain; and E/F, ligand-binding domain. The site of the Neo gene insertion is indicated by the open arrow.