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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: Anal Chem. 2009 Jul 15;81(14):5858–5864. doi: 10.1021/ac900936g

Figure 6.

Figure 6

Analyte characterization and identification by single cell CE-ESI-MS. (a) Confirmation of 5-HT in a single MCC neuron. Migration time and mass information for the putative 5-HT peak in an MCC neuron matches the information obtained from analyzing a 5-HT standard solution. (b) Metabolite characterization via MS/MS. Different isobaric compounds (m/z = 146.1) present in a single R2 neuron are subjected to MS/MS analysis as they elute from the CE capillary using a Bruker maXis mass spectrometer. The peak at 15.4 min yields a fragment at 87 m/z, which is consistent with ACh, while other compounds either do not fragment appreciably, or yield fragments that are inconsistent with ACh. The peak corresponding to ACh was also confirmed by sample spiking (data not shown).