Figure 1. Effects of MC1575 and MC1568 on deacetylase activity in MCF-7 cells.
MCF-7 cells were treated for 6 h with TSA (1.7 μM), MC1575 (20 μM), MC1568 (20 μM) or MS-275 (1 μM). Cell lysates were analyzed by western immunoblotting using anti-acetylated histone H3 (Acetyl-H3), anti-acetylated histone H4 (Acetyl-H4) or anti-acetylated tubulin (Ac-tubulin) antibodies. Actin was used as a loading control.