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. Author manuscript; available in PMC: 2010 Jan 25.
Published in final edited form as: Mol Cancer Res. 2008 Dec;6(12):1908–1919. doi: 10.1158/1541-7786.MCR-08-0299

Figure 6. Effects of class II HDI on the expression of cell cycle and apoptosis regulators.

Figure 6

A- MCF-7 cells were treated with TSA (1.7 μM), MC1575 (20 μM) or vehicle alone (Control - Ctl) for 6 h (p27 and p14ARF) or 20 h (p21cip1/waf1 and cyclin D1) and mRNA levels for p21, cyclin D1, p27 and p14 genes were measured using RT-qPCR. Results are expressed relative to the TBP housekeeping gene and to the mRNA levels measured for the untreated control cells used as reference. Results represent mean and s.d of 4 independent cell cultures. For p21 and cyclin D1, western-blot analysis was performed in the same conditions, using actin as a loading control.

B- MCF-7 cells were treated for 20h with TSA (1.7 μM), MC1575 (20 μM) or vehicle alone (Control - Ctl) and Bcl2, Bax α, TRAIL-R1 and TRAIL-R2 mRNA levels were quantified using RT-qPCR. Results were expressed as in A and represent mean and s.d of 4 independent cell cultures. Raw data were used for statistical analysis, §p = 0.05, * p < 0.05 as compared to control cells.