Skip to main content
. 2010 Jan 25;5(1):e8863. doi: 10.1371/journal.pone.0008863

Figure 6. DNA synapsis and cleavage assays with wild-type and mutant integrases.

Figure 6

Labelled attB was incubated with wild-type or mutant integrases (Panel A:R18A, I141A; Panel B:L143A, E153A; Panel C:I432A) in the presence of no cold attP, 198 bp cold attP in binding buffer and then untreated or treated with proteinase K. Reactions were analyzed by polyacrylamide gel electrophoresis (5% polyacrylamide, 1x Tris-borate-EDTA). The complexes observed are integrases (Int) bound to attB or synaptic complexes (SC). The recombinant products attL and attR are also indicated.