Figure 4. PME-1 supports malignant cell growth.
(A) Dense foci formation of HeLa cells transfected with scrambled or PME-1 siRNAs. (B) Anchorage-independent growth of HeLa cells transfected with scrambled or PME-1 siRNA. (A, B) Colony numbers were analysed by Image J software. Data is presented as percentual change of number of colonies as compared to Scr. siRNA transfected cells which were set in each experiment as 100%. Shown is mean + S.D. of 4-5 experiments. *p= 0.001, *p = 0.002, Student's t test. (C) Proliferation of HeLa cells transfected with scrambled or PME-1 siRNA or treated with UO126 MEK inhibitor for 48-72 hours. Shown is a mean value +S.D. of 2 replicate plates. Experiment was performed 3 times with similar results. *p<0.05, **p<0.01, Student's t test. (D) Effect of PME-1 depletion on proliferation rates of indicated HEK-TE cells. The data is presented as mean + S.D. of 3 independent experiments. **p< 0.01, n.s.= not significant.